KMID : 1098420090170010068
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Korean Journal of Medicinal Crop Science 2009 Volume.17 No. 1 p.68 ~ p.74
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Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons
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Kim Ju-Yeon
Ju Hyun-Soo Ban Joo-Yean Song Kyung-Sik Bae Ki-Hwan Seong Yeon-Hee
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Abstract
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Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (H2O2) (100 ¥ìM)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100 ¥ìg/ml) concentration-dependently inhibited H2O2-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited H2O2-induced elevation of intracellular Ca2+ concentration ([Ca2+]i) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100 ¥ìM H2O2, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on H2O2-induced neuronal cell death by interfering with H2O2-induced elevation of [Ca2+]i, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.
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KEYWORD
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Vitis amurensis, Hydrogen Peroxide, Neurotoxicity, Neuroprotection, Anti-oxidant
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